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Comment by zulko

9 hours ago

Possibly not what you're asking for, but I wrote a generally-accessible intro to why it can be tricky to assemble many DNA fragments with "Golden Gate Assembly", a mainstream method which relies on short sequence overhangs. The Sidewinder method discussed in this thread aims to solve that "short overhang" problem.

https://zulko.github.io/bricks_and_scissors/posts/overhangs/

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