Comment by mbreese

I don’t think PCR is necessarily relevant here. I had the impression that this would be lost useful at linking multi-kb fragments together. If we are looking at sizes much above 2kb, PCR is going to struggle to generate full length fragments efficiently.

I didn’t see this technique as having DNA modification per-se, but a novel way to managing the hybridization process. It’s stock (well engineered) oligos, if I read it correctly.