Comment by dnautics
11 hours ago
iiuc it was hamilton smith who insisted that shotgun sequencing would work. the nih side insisted on primer walking until celera started assembling the genome so rapidly that the nih had to get in on shotgun too
11 hours ago
iiuc it was hamilton smith who insisted that shotgun sequencing would work. the nih side insisted on primer walking until celera started assembling the genome so rapidly that the nih had to get in on shotgun too
I belive you are mixing assembling the genome by combining sequences of individual, overlapping inserts of cosmids, fosmids, PACs and BACs (bacterial vectors with human DNA inserts of 40-150kbp) to whole genome shotgun. The inserts of the above bacterial vectors were sequenced using shotgun, but the gaps in the sequence were closed with custom primers.
No, at initial release, the human genome from the NIH side was done by bac-to-bac, not by shotgun.